{"id":10733,"date":"2021-08-06T14:59:40","date_gmt":"2021-08-06T12:59:40","guid":{"rendered":"https:\/\/www.endoscopy-campus.com\/?post_type=ec-news&#038;p=10733"},"modified":"2021-08-06T14:59:41","modified_gmt":"2021-08-06T12:59:41","slug":"dna-methylation-studies-of-biliary-brush-cytology-samples-likely-to-aid-in-diagnosis-of-cholangiocarcinoma","status":"publish","type":"ec-news","link":"https:\/\/www.endoscopy-campus.com\/en\/ec-news\/dna-methylation-studies-of-biliary-brush-cytology-samples-likely-to-aid-in-diagnosis-of-cholangiocarcinoma\/","title":{"rendered":"DNA Methylation Studies of Biliary Brush Cytology Samples Likely to Aid in Diagnosis of Cholangiocarcinoma"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">Establishing a firm diagnosis for indeterminate biliary strictures is often challenging. The current standard of practice is fluoroscopic and cholangioscopic characterization with brush and biopsy sampling for cytology, histology, and fluorescent in situ hybridization (FISH) evaluation, which are highly specific but relatively insensitive for cholangiocarcinoma (CCA). Advanced endoscopic and cellular\/pathology assessments have been described. This single-center study sought to determine methylation indices (MIs) for 2 gene promoters (<em>HOXA1<\/em>&nbsp;and&nbsp;<em>NEUROG1<\/em>) of CCA by prospectively performing polymerase chain reaction assessment of DNA extracted from brush cytology specimens acquired at ERCP.&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Among 67 patients, 41 were subsequently confirmed to have CCA, and 26 had benign strictures, with equal distributions by age and gender. The following sensitivity and accuracy data were obtained for the MIs and brush cytology and serum CA 19-9 (&gt;100) levels:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">MI<em>&nbsp;HOXA1<\/em>: &nbsp; &nbsp;95.1%, 82.3%&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">MI&nbsp;<em>NEUROG1<\/em>:&nbsp; &nbsp;90.2%, 89.5%<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Brush cytology:&nbsp; 61.5%, 78.1%&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">CA 19-9:&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;69.4%, 77.8%<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">The combination of both MI tests, cytology, and CA 19-9 yielded maximum sensitivity of 97.4%, with associated specificity of 82.4% and accuracy of 91%. Cytology alone was most specific (100%), and MIs plus cytology was most accurate (92.4%). Among 6 patients with confirmed CCA but negative CA 19-9 and cytology, the MIs were positive in 5.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Establishing a firm diagnosis for indeterminate biliary strictures is often challenging. The current standard of practice is fluoroscopic and cholangioscopic characterization with brush and biopsy sampling for cytology, histology, and fluorescent in situ hybridization (FISH) evaluation, which are highly specific but relatively insensitive for cholangiocarcinoma (CCA). Advanced endoscopic and cellular\/pathology assessments have been described. This &#8230; <a title=\"DNA Methylation Studies of Biliary Brush Cytology Samples Likely to Aid in Diagnosis of Cholangiocarcinoma\" class=\"read-more\" href=\"https:\/\/www.endoscopy-campus.com\/en\/ec-news\/dna-methylation-studies-of-biliary-brush-cytology-samples-likely-to-aid-in-diagnosis-of-cholangiocarcinoma\/\" aria-label=\"Read more about DNA Methylation Studies of Biliary Brush Cytology Samples Likely to Aid in Diagnosis of Cholangiocarcinoma\">Read more<\/a><\/p>\n","protected":false},"template":"","categories":[1768,67],"tags":[2877,2876,2875,2874,2873,2128],"class_list":["post-10733","ec-news","type-ec-news","status-publish","hentry","category-asge-journal-scan","category-bauchspeicheldruese-pankreas","tag-ca-19-9","tag-cytology-2","tag-dna-methylation","tag-fish","tag-fluorescent-in-situ-hybridization","tag-pancreatobiliary"],"_links":{"self":[{"href":"https:\/\/www.endoscopy-campus.com\/en\/wp-json\/wp\/v2\/ec-news\/10733","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.endoscopy-campus.com\/en\/wp-json\/wp\/v2\/ec-news"}],"about":[{"href":"https:\/\/www.endoscopy-campus.com\/en\/wp-json\/wp\/v2\/types\/ec-news"}],"wp:attachment":[{"href":"https:\/\/www.endoscopy-campus.com\/en\/wp-json\/wp\/v2\/media?parent=10733"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.endoscopy-campus.com\/en\/wp-json\/wp\/v2\/categories?post=10733"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.endoscopy-campus.com\/en\/wp-json\/wp\/v2\/tags?post=10733"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}