Currently, an upper GI endoscopy with biopsies of the columnar-lined distal esophagus is necessary to diagnose Barrett\u2019s esophagus (BE). Easier, noninvasive, nonendoscopy-based testing techniques have been identified for this precancerous condition. This prospective case-control study evaluated the performance of methylated DNA markers (MDMs) for detecting BE from mucosal cells obtained by a swallowed device.<\/p>\n\n\n\n
Subjects with and without BE (cases vs controls) swallowed a sponge-on-string (SOS) device that was withdrawn after 8 minutes of ingestion. The cells from the sponge were collected, and DNA was extracted and evaluated for a panel of MDMs (ZNF568<\/em>, BMP3<\/em>, NDRG4<\/em>, VAV3<\/em>, ZNF682<\/em>). The study included 199 subjects (110 cases: 83% male, mean age of 62 years, and 89 controls: 47% male, mean age of 60 years) in a training set to calibrate the MDM panel and, thereafter, 60 cases (83% male, mean age of 65 years) and 29 controls (48% male, mean age of 62 years) in a test set to evaluate the MDM panel against the current gold standard (upper endoscopy and biopsy). <\/p>\n\n\n\n The sensitivity, specificity, and area under the curve (AUC) were 93%, 90%, and 96%, respectively, for the training set and 93%, 93%, and 97%, respectively, for the test set. At a prespecified specificity of 90% in the test set, the 5-MDM panel showed a sensitivity of 94%, 96%, and 86% to 100% for the detection of nondysplastic BE, any BE dysplasia, and BE neoplasia (high-grade dysplasia or early esophageal adenocarcinoma). The authors performed a 3-panel MDM (10 possible combinations of 3 MDMs) as well, and the specificity, sensitivity, and AUC were 92% to 93%, 89% to 92%, and 95% to 97%, respectively. The patients rated the tolerability of the SOS device-based procedure at a mean score of 2.2 (score range 0-10), and no major complications were noted.<\/p>\n","protected":false},"excerpt":{"rendered":" Currently, an upper GI endoscopy with biopsies of the columnar-lined distal esophagus is necessary to diagnose Barrett\u2019s esophagus (BE). Easier, noninvasive, nonendoscopy-based testing techniques have been identified for this precancerous condition. This prospective case-control study evaluated the performance of methylated DNA markers (MDMs) for detecting BE from mucosal cells obtained by a swallowed device. Subjects … Read more<\/a><\/p>\n","protected":false},"template":"","categories":[1768,1791],"tags":[2098,2744,2743,2745],"class_list":["post-10414","ec-news","type-ec-news","status-publish","hentry","category-asge-journal-scan","category-esophagus","tag-esophagus-2","tag-mdms","tag-methylated-dna-markers","tag-nonendoscopy-based-testing-techniques"],"_links":{"self":[{"href":"https:\/\/www.endoscopy-campus.com\/en\/wp-json\/wp\/v2\/ec-news\/10414","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.endoscopy-campus.com\/en\/wp-json\/wp\/v2\/ec-news"}],"about":[{"href":"https:\/\/www.endoscopy-campus.com\/en\/wp-json\/wp\/v2\/types\/ec-news"}],"wp:attachment":[{"href":"https:\/\/www.endoscopy-campus.com\/en\/wp-json\/wp\/v2\/media?parent=10414"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.endoscopy-campus.com\/en\/wp-json\/wp\/v2\/categories?post=10414"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.endoscopy-campus.com\/en\/wp-json\/wp\/v2\/tags?post=10414"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}